Construct: shRNA TRCN0000357566

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_175911.2-685s21c1
DNA Barcode:
CATGGCCTACGACCGTTATTT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
OR2L13 (284521)
Gene Description:
olfactory receptor family 2 subfamily L member 13
Transcript:
RefSeq NM_175911.2 (NON-CURRENT)
Match location:
Position 685 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000357566)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CATGGCCTACGACCGTTATTT
Hairpin Sequence:
5'-CCGG-CATGGCCTACGACCGTTATTT-CTCGAG-AAATAACGGTCGTAGGCCATG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCATGGCCTACGACCGTTATTTCTCGAGAAATAACGGTCGTAGGCCATGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACATGGCCTACGACCGTTATTTCTCGAGAAATAACGGTCGTAGGCCATG-3'

Other clones with same target sequence:

(none)