Construct: shRNA TRCN0000357602

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_019839.3-2851s21c1
DNA Barcode:
GTCAGTGTTCTGGGACATTTG

Original Target:

Taxon:
Homo sapiens (human)
Gene:
LTB4R2 (56413)
Gene Description:
leukotriene B4 receptor 2
Transcript:
RefSeq NM_019839.3 (NON-CURRENT)
Match location:
Position 2851 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000357602)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GTCAGTGTTCTGGGACATTTG
Hairpin Sequence:
5'-CCGG-GTCAGTGTTCTGGGACATTTG-CTCGAG-CAAATGTCCCAGAACACTGAC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGTCAGTGTTCTGGGACATTTGCTCGAGCAAATGTCCCAGAACACTGACTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGTCAGTGTTCTGGGACATTTGCTCGAGCAAATGTCCCAGAACACTGAC-3'

Other clones with same target sequence:

(none)