Construct: shRNA TRCN0000357742

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_030908.1-631s21c1
DNA Barcode:
TCCACAATTGTAGTTTCATAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
OR2A4 (79541)
Gene Description:
olfactory receptor family 2 subfamily A member 4
Transcript:
RefSeq NM_030908.1 (NON-CURRENT)
Match location:
Position 631 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000357742)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TCCACAATTGTAGTTTCATAT
Hairpin Sequence:
5'-CCGG-TCCACAATTGTAGTTTCATAT-CTCGAG-ATATGAAACTACAATTGTGGA-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTCCACAATTGTAGTTTCATATCTCGAGATATGAAACTACAATTGTGGATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATCCACAATTGTAGTTTCATATCTCGAGATATGAAACTACAATTGTGGA-3'

Other clones with same target sequence:

(none)