Construct: shRNA TRCN0000357826

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_007160.3-543s21c1
DNA Barcode:
CGATCGGCAGGTGGATGATTT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
OR2H2 (7932)
Gene Description:
olfactory receptor family 2 subfamily H member 2
Transcript:
RefSeq NM_007160.3 (CURRENT)
Match location:
Position 543 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000357826)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CGATCGGCAGGTGGATGATTT
Hairpin Sequence:
5'-CCGG-CGATCGGCAGGTGGATGATTT-CTCGAG-AAATCATCCACCTGCCGATCG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCGATCGGCAGGTGGATGATTTCTCGAGAAATCATCCACCTGCCGATCGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACGATCGGCAGGTGGATGATTTCTCGAGAAATCATCCACCTGCCGATCG-3'

Other clones with same target sequence:

(none)