Construct: shRNA TRCN0000357881

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_022146.2-89s21c1
DNA Barcode:
TCACCTTCTCCTCCTACTATC

Original Target:

Taxon:
Homo sapiens (human)
Gene:
NPFFR1 (64106)
Gene Description:
neuropeptide FF receptor 1
Transcript:
RefSeq NM_022146.2 (NON-CURRENT)
Match location:
Position 89 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000357881)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TCACCTTCTCCTCCTACTATC
Hairpin Sequence:
5'-CCGG-TCACCTTCTCCTCCTACTATC-CTCGAG-GATAGTAGGAGGAGAAGGTGA-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTCACCTTCTCCTCCTACTATCCTCGAGGATAGTAGGAGGAGAAGGTGATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATCACCTTCTCCTCCTACTATCCTCGAGGATAGTAGGAGGAGAAGGTGA-3'

Other clones with same target sequence:

(none)