Construct: shRNA TRCN0000357883

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_000916.3-2072s21c1
DNA Barcode:
GTAATTTCACTCCAGTATATT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
OXTR (5021)
Gene Description:
oxytocin receptor
Transcript:
RefSeq NM_000916.3 (CURRENT)
Match location:
Position 2072 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000357883)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GTAATTTCACTCCAGTATATT
Hairpin Sequence:
5'-CCGG-GTAATTTCACTCCAGTATATT-CTCGAG-AATATACTGGAGTGAAATTAC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGTAATTTCACTCCAGTATATTCTCGAGAATATACTGGAGTGAAATTACTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGTAATTTCACTCCAGTATATTCTCGAGAATATACTGGAGTGAAATTAC-3'

Other clones with same target sequence:

(none)