Construct: shRNA TRCN0000358149

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_000369.2-2648s21c1
DNA Barcode:
GTTAGGCTACCAGCATATTTG

Original Target:

Taxon:
Homo sapiens (human)
Gene:
TSHR (7253)
Gene Description:
thyroid stimulating hormone receptor
Transcript:
RefSeq NM_000369.2 (NON-CURRENT)
Match location:
Position 2648 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000358149)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GTTAGGCTACCAGCATATTTG
Hairpin Sequence:
5'-CCGG-GTTAGGCTACCAGCATATTTG-CTCGAG-CAAATATGCTGGTAGCCTAAC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGTTAGGCTACCAGCATATTTGCTCGAGCAAATATGCTGGTAGCCTAACTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGTTAGGCTACCAGCATATTTGCTCGAGCAAATATGCTGGTAGCCTAAC-3'

Other clones with same target sequence:

(none)