Construct: shRNA TRCN0000358250

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_004736.3-1317s21c1
DNA Barcode:
TAAACTGCTGTTTCGAGTATT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
XPR1 (9213)
Gene Description:
xenotropic and polytropic retrovirus receptor 1
Transcript:
RefSeq NM_004736.3 (NON-CURRENT)
Match location:
Position 1317 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000358250)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TAAACTGCTGTTTCGAGTATT
Hairpin Sequence:
5'-CCGG-TAAACTGCTGTTTCGAGTATT-CTCGAG-AATACTCGAAACAGCAGTTTA-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTAAACTGCTGTTTCGAGTATTCTCGAGAATACTCGAAACAGCAGTTTATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATAAACTGCTGTTTCGAGTATTCTCGAGAATACTCGAAACAGCAGTTTA-3'

Other clones with same target sequence:

(none)