Construct: shRNA TRCN0000358416

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001161808.1-1919s21c1
DNA Barcode:
CCATCCTGCTGCAAATCTATG

Original Target:

Taxon:
Homo sapiens (human)
Gene:
ADGRD2 (347088)
Gene Description:
adhesion G protein-coupled receptor D2
Transcript:
RefSeq NM_001161808.1 (NON-CURRENT)
Match location:
Position 1919 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000358416)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:


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Sequence Information

Target Sequence:
CCATCCTGCTGCAAATCTATG
Hairpin Sequence:
5'-CCGG-CCATCCTGCTGCAAATCTATG-CTCGAG-CATAGATTTGCAGCAGGATGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCATCCTGCTGCAAATCTATGCTCGAGCATAGATTTGCAGCAGGATGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCATCCTGCTGCAAATCTATGCTCGAGCATAGATTTGCAGCAGGATGG-3'

Other clones with same target sequence:

(none)