Construct: shRNA TRCN0000358527

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_018489.2-5046s21c1
DNA Barcode:
TGCTGTTGGAGAGCGATATAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
ASH1L (55870)
Gene Description:
ASH1 like histone lysine methyltransferase
Transcript:
RefSeq NM_018489.2 (NON-CURRENT)
Match location:
Position 5046 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000358527)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TGCTGTTGGAGAGCGATATAA
Hairpin Sequence:
5'-CCGG-TGCTGTTGGAGAGCGATATAA-CTCGAG-TTATATCGCTCTCCAACAGCA-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTGCTGTTGGAGAGCGATATAACTCGAGTTATATCGCTCTCCAACAGCATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATGCTGTTGGAGAGCGATATAACTCGAGTTATATCGCTCTCCAACAGCA-3'

Other clones with same target sequence:

(none)