Construct: shRNA TRCN0000358690

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_003300.2-707s21c1
DNA Barcode:
CACGTGGAGAAGGCGTGTAAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
TRAF3 (7187)
Gene Description:
TNF receptor associated factor 3
Transcript:
RefSeq NM_003300.2 (NON-CURRENT)
Match location:
Position 707 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000358690)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CACGTGGAGAAGGCGTGTAAA
Hairpin Sequence:
5'-CCGG-CACGTGGAGAAGGCGTGTAAA-CTCGAG-TTTACACGCCTTCTCCACGTG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCACGTGGAGAAGGCGTGTAAACTCGAGTTTACACGCCTTCTCCACGTGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACACGTGGAGAAGGCGTGTAAACTCGAGTTTACACGCCTTCTCCACGTG-3'

Other clones with same target sequence:

(none)