Construct: shRNA TRCN0000358717

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_003156.3-1215s21c1
DNA Barcode:
TGGTGGTGTCTATCGTTATTG

Original Target:

Taxon:
Homo sapiens (human)
Gene:
STIM1 (6786)
Gene Description:
stromal interaction molecule 1
Transcript:
RefSeq NM_003156.3 (CURRENT)
Match location:
Position 1215 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000358717)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TGGTGGTGTCTATCGTTATTG
Hairpin Sequence:
5'-CCGG-TGGTGGTGTCTATCGTTATTG-CTCGAG-CAATAACGATAGACACCACCA-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTGGTGGTGTCTATCGTTATTGCTCGAGCAATAACGATAGACACCACCATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATGGTGGTGTCTATCGTTATTGCTCGAGCAATAACGATAGACACCACCA-3'

Other clones with same target sequence:

(none)