Construct: shRNA TRCN0000358754

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_152428.4-1897s21c1
DNA Barcode:
ATTCAGCCAAGACCAGTAAAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
FRMPD2 (143162)
Gene Description:
FERM and PDZ domain containing 2
Transcript:
RefSeq NM_152428.4 (NON-CURRENT)
Match location:
Position 1897 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000358754)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
ATTCAGCCAAGACCAGTAAAT
Hairpin Sequence:
5'-CCGG-ATTCAGCCAAGACCAGTAAAT-CTCGAG-ATTTACTGGTCTTGGCTGAAT-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGATTCAGCCAAGACCAGTAAATCTCGAGATTTACTGGTCTTGGCTGAATTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAATTCAGCCAAGACCAGTAAATCTCGAGATTTACTGGTCTTGGCTGAAT-3'

Other clones with same target sequence:

(none)