Construct: shRNA TRCN0000358793

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_032415.3-1578s21c1
DNA Barcode:
CAGTACTCGCAGTGCTTAATC

Original Target:

Taxon:
Homo sapiens (human)
Gene:
CARD11 (84433)
Gene Description:
caspase recruitment domain family member 11
Transcript:
RefSeq NM_032415.3 (NON-CURRENT)
Match location:
Position 1578 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000358793)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CAGTACTCGCAGTGCTTAATC
Hairpin Sequence:
5'-CCGG-CAGTACTCGCAGTGCTTAATC-CTCGAG-GATTAAGCACTGCGAGTACTG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCAGTACTCGCAGTGCTTAATCCTCGAGGATTAAGCACTGCGAGTACTGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACAGTACTCGCAGTGCTTAATCCTCGAGGATTAAGCACTGCGAGTACTG-3'

Other clones with same target sequence:

(none)