Construct: shRNA TRCN0000358826

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_006573.3-721s21c1
DNA Barcode:
GACAGTGAAACACCAACTATA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
TNFSF13B (10673)
Gene Description:
TNF superfamily member 13b
Transcript:
RefSeq NM_006573.3 (NON-CURRENT)
Match location:
Position 721 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000358826)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GACAGTGAAACACCAACTATA
Hairpin Sequence:
5'-CCGG-GACAGTGAAACACCAACTATA-CTCGAG-TATAGTTGGTGTTTCACTGTC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGACAGTGAAACACCAACTATACTCGAGTATAGTTGGTGTTTCACTGTCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGACAGTGAAACACCAACTATACTCGAGTATAGTTGGTGTTTCACTGTC-3'

Other clones with same target sequence:

(none)