Construct: shRNA TRCN0000358877

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_032329.4-792s21c1
DNA Barcode:
CTTCATTCGTGTCGCAATATT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
ING5 (84289)
Gene Description:
inhibitor of growth family member 5
Transcript:
RefSeq NM_032329.4 (NON-CURRENT)
Match location:
Position 792 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000358877)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CTTCATTCGTGTCGCAATATT
Hairpin Sequence:
5'-CCGG-CTTCATTCGTGTCGCAATATT-CTCGAG-AATATTGCGACACGAATGAAG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCTTCATTCGTGTCGCAATATTCTCGAGAATATTGCGACACGAATGAAGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACTTCATTCGTGTCGCAATATTCTCGAGAATATTGCGACACGAATGAAG-3'

Other clones with same target sequence:

(none)