Construct: shRNA TRCN0000358888

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_178844.2-3335s21c1
DNA Barcode:
CCTTGGAGATTCTCGACTTAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
NLRC3 (197358)
Gene Description:
NLR family CARD domain containing 3
Transcript:
RefSeq NM_178844.2 (NON-CURRENT)
Match location:
Position 3335 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000358888)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCTTGGAGATTCTCGACTTAA
Hairpin Sequence:
5'-CCGG-CCTTGGAGATTCTCGACTTAA-CTCGAG-TTAAGTCGAGAATCTCCAAGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCTTGGAGATTCTCGACTTAACTCGAGTTAAGTCGAGAATCTCCAAGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCTTGGAGATTCTCGACTTAACTCGAGTTAAGTCGAGAATCTCCAAGG-3'

Other clones with same target sequence:

(none)