Construct: shRNA TRCN0000358917

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_005393.2-308s21c1
DNA Barcode:
ATCGCTTCTCCGCACCTAATA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
PLXNB3 (5365)
Gene Description:
plexin B3
Transcript:
RefSeq NM_005393.2 (NON-CURRENT)
Match location:
Position 308 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000358917)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
ATCGCTTCTCCGCACCTAATA
Hairpin Sequence:
5'-CCGG-ATCGCTTCTCCGCACCTAATA-CTCGAG-TATTAGGTGCGGAGAAGCGAT-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGATCGCTTCTCCGCACCTAATACTCGAGTATTAGGTGCGGAGAAGCGATTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAATCGCTTCTCCGCACCTAATACTCGAGTATTAGGTGCGGAGAAGCGAT-3'

Other clones with same target sequence:

(none)