Construct: shRNA TRCN0000358920

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_022336.3-1382s21c1
DNA Barcode:
CAAGTCAGCCGGGATTCAAAG

Original Target:

Taxon:
Homo sapiens (human)
Gene:
EDAR (10913)
Gene Description:
ectodysplasin A receptor
Transcript:
RefSeq NM_022336.3 (NON-CURRENT)
Match location:
Position 1382 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000358920)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CAAGTCAGCCGGGATTCAAAG
Hairpin Sequence:
5'-CCGG-CAAGTCAGCCGGGATTCAAAG-CTCGAG-CTTTGAATCCCGGCTGACTTG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCAAGTCAGCCGGGATTCAAAGCTCGAGCTTTGAATCCCGGCTGACTTGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACAAGTCAGCCGGGATTCAAAGCTCGAGCTTTGAATCCCGGCTGACTTG-3'

Other clones with same target sequence:

(none)