Construct: shRNA TRCN0000359103

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_000540.2-14099s21c1
DNA Barcode:
CTCTGCATCATTGGCTATAAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
RYR1 (6261)
Gene Description:
ryanodine receptor 1
Transcript:
RefSeq NM_000540.2 (NON-CURRENT)
Match location:
Position 14099 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000359103)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CTCTGCATCATTGGCTATAAT
Hairpin Sequence:
5'-CCGG-CTCTGCATCATTGGCTATAAT-CTCGAG-ATTATAGCCAATGATGCAGAG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCTCTGCATCATTGGCTATAATCTCGAGATTATAGCCAATGATGCAGAGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACTCTGCATCATTGGCTATAATCTCGAGATTATAGCCAATGATGCAGAG-3'

Other clones with same target sequence:

(none)