Construct: shRNA TRCN0000359107

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_014977.3-1108s21c1
DNA Barcode:
GGTCAGAGCAGATCGAAATTT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
ACIN1 (22985)
Gene Description:
apoptotic chromatin condensation inducer 1
Transcript:
RefSeq NM_014977.3 (CURRENT)
Match location:
Position 1108 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000359107)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GGTCAGAGCAGATCGAAATTT
Hairpin Sequence:
5'-CCGG-GGTCAGAGCAGATCGAAATTT-CTCGAG-AAATTTCGATCTGCTCTGACC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGGTCAGAGCAGATCGAAATTTCTCGAGAAATTTCGATCTGCTCTGACCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGGTCAGAGCAGATCGAAATTTCTCGAGAAATTTCGATCTGCTCTGACC-3'

Other clones with same target sequence:

(none)