Construct: shRNA TRCN0000359140

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_004242.2-457s21c1
DNA Barcode:
TGTAGATAACGAGGGAGAATG

Original Target:

Taxon:
Homo sapiens (human)
Gene:
HMGN3 (9324)
Gene Description:
high mobility group nucleosomal binding domain 3
Transcript:
RefSeq NM_004242.2 (NON-CURRENT)
Match location:
Position 457 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000359140)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TGTAGATAACGAGGGAGAATG
Hairpin Sequence:
5'-CCGG-TGTAGATAACGAGGGAGAATG-CTCGAG-CATTCTCCCTCGTTATCTACA-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTGTAGATAACGAGGGAGAATGCTCGAGCATTCTCCCTCGTTATCTACATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATGTAGATAACGAGGGAGAATGCTCGAGCATTCTCCCTCGTTATCTACA-3'

Other clones with same target sequence:

(none)