Construct: shRNA TRCN0000359255

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_012403.1-249s21c1
DNA Barcode:
TCCAAACCTCACGCATCTATA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
ANP32C (23520)
Gene Description:
acidic nuclear phosphoprotein 32 family member C
Transcript:
RefSeq NM_012403.1 (CURRENT)
Match location:
Position 249 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000359255)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TCCAAACCTCACGCATCTATA
Hairpin Sequence:
5'-CCGG-TCCAAACCTCACGCATCTATA-CTCGAG-TATAGATGCGTGAGGTTTGGA-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTCCAAACCTCACGCATCTATACTCGAGTATAGATGCGTGAGGTTTGGATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATCCAAACCTCACGCATCTATACTCGAGTATAGATGCGTGAGGTTTGGA-3'

Other clones with same target sequence:

(none)