Construct: shRNA TRCN0000359266

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_000122.1-2416s21c1
DNA Barcode:
ACCCGCTCTTCAAGCGCTTTA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
ERCC3 (2071)
Gene Description:
ERCC excision repair 3, TFIIH core complex helicase subunit
Transcript:
RefSeq NM_000122.1 (NON-CURRENT)
Match location:
Position 2416 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000359266)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
ACCCGCTCTTCAAGCGCTTTA
Hairpin Sequence:
5'-CCGG-ACCCGCTCTTCAAGCGCTTTA-CTCGAG-TAAAGCGCTTGAAGAGCGGGT-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGACCCGCTCTTCAAGCGCTTTACTCGAGTAAAGCGCTTGAAGAGCGGGTTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAACCCGCTCTTCAAGCGCTTTACTCGAGTAAAGCGCTTGAAGAGCGGGT-3'

Other clones with same target sequence:

(none)