Construct: shRNA TRCN0000359272

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_020126.3-911s21c1
DNA Barcode:
GGTTGCTTCTATTGGTCAATC

Original Target:

Taxon:
Homo sapiens (human)
Gene:
SPHK2 (56848)
Gene Description:
sphingosine kinase 2
Transcript:
RefSeq NM_020126.3 (NON-CURRENT)
Match location:
Position 911 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000359272)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GGTTGCTTCTATTGGTCAATC
Hairpin Sequence:
5'-CCGG-GGTTGCTTCTATTGGTCAATC-CTCGAG-GATTGACCAATAGAAGCAACC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGGTTGCTTCTATTGGTCAATCCTCGAGGATTGACCAATAGAAGCAACCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGGTTGCTTCTATTGGTCAATCCTCGAGGATTGACCAATAGAAGCAACC-3'

Other clones with same target sequence:

(none)