Construct: shRNA TRCN0000359334

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_021140.2-3564s21c1
DNA Barcode:
TGAATCTACATCGTCAGATAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
KDM6A (7403)
Gene Description:
lysine demethylase 6A
Transcript:
RefSeq NM_021140.2 (NON-CURRENT)
Match location:
Position 3564 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000359334)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TGAATCTACATCGTCAGATAA
Hairpin Sequence:
5'-CCGG-TGAATCTACATCGTCAGATAA-CTCGAG-TTATCTGACGATGTAGATTCA-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTGAATCTACATCGTCAGATAACTCGAGTTATCTGACGATGTAGATTCATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATGAATCTACATCGTCAGATAACTCGAGTTATCTGACGATGTAGATTCA-3'

Other clones with same target sequence:

(none)