Construct: shRNA TRCN0000359419

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_005637.2-774s21c1
DNA Barcode:
GGGAATGATGGGTCAAGTTAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
SS18 (6760)
Gene Description:
SS18 subunit of BAF chromatin remodeling complex
Transcript:
RefSeq NM_005637.2 (NON-CURRENT)
Match location:
Position 774 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000359419)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GGGAATGATGGGTCAAGTTAA
Hairpin Sequence:
5'-CCGG-GGGAATGATGGGTCAAGTTAA-CTCGAG-TTAACTTGACCCATCATTCCC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGGGAATGATGGGTCAAGTTAACTCGAGTTAACTTGACCCATCATTCCCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGGGAATGATGGGTCAAGTTAACTCGAGTTAACTTGACCCATCATTCCC-3'

Other clones with same target sequence:

(none)