Construct: shRNA TRCN0000359443

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_080822.2-552s21c1
DNA Barcode:
CTGCAAAGGCCCTTGTCATTG

Original Target:

Taxon:
Homo sapiens (human)
Gene:
OVCA2 (124641)
Gene Description:
OVCA2 serine hydrolase domain containing
Transcript:
RefSeq NM_080822.2 (NON-CURRENT)
Match location:
Position 552 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000359443)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CTGCAAAGGCCCTTGTCATTG
Hairpin Sequence:
5'-CCGG-CTGCAAAGGCCCTTGTCATTG-CTCGAG-CAATGACAAGGGCCTTTGCAG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCTGCAAAGGCCCTTGTCATTGCTCGAGCAATGACAAGGGCCTTTGCAGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACTGCAAAGGCCCTTGTCATTGCTCGAGCAATGACAAGGGCCTTTGCAG-3'

Other clones with same target sequence:

(none)