Construct: shRNA TRCN0000359634

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_175842.1-655s21c1
DNA Barcode:
GAGTTCTTCCGGCACGATAAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
SMOX (54498)
Gene Description:
spermine oxidase
Transcript:
RefSeq NM_175842.1 (NON-CURRENT)
Match location:
Position 655 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000359634)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GAGTTCTTCCGGCACGATAAA
Hairpin Sequence:
5'-CCGG-GAGTTCTTCCGGCACGATAAA-CTCGAG-TTTATCGTGCCGGAAGAACTC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGAGTTCTTCCGGCACGATAAACTCGAGTTTATCGTGCCGGAAGAACTCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGAGTTCTTCCGGCACGATAAACTCGAGTTTATCGTGCCGGAAGAACTC-3'

Other clones with same target sequence:

(none)