Construct: shRNA TRCN0000359742

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_022140.3-391s21c1
DNA Barcode:
CGTTGTCCTTGACCACGTATT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
EPB41L4A (64097)
Gene Description:
erythrocyte membrane protein band 4.1 like 4A
Transcript:
RefSeq NM_022140.3 (NON-CURRENT)
Match location:
Position 391 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000359742)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CGTTGTCCTTGACCACGTATT
Hairpin Sequence:
5'-CCGG-CGTTGTCCTTGACCACGTATT-CTCGAG-AATACGTGGTCAAGGACAACG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCGTTGTCCTTGACCACGTATTCTCGAGAATACGTGGTCAAGGACAACGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACGTTGTCCTTGACCACGTATTCTCGAGAATACGTGGTCAAGGACAACG-3'

Other clones with same target sequence:

(none)