Construct: shRNA TRCN0000359743

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_024110.2-2730s21c1
DNA Barcode:
GTGCCTCCTCCAAGGGTTTAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
CARD14 (79092)
Gene Description:
caspase recruitment domain family member 14
Transcript:
RefSeq NM_024110.2 (NON-CURRENT)
Match location:
Position 2730 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000359743)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GTGCCTCCTCCAAGGGTTTAA
Hairpin Sequence:
5'-CCGG-GTGCCTCCTCCAAGGGTTTAA-CTCGAG-TTAAACCCTTGGAGGAGGCAC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGTGCCTCCTCCAAGGGTTTAACTCGAGTTAAACCCTTGGAGGAGGCACTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGTGCCTCCTCCAAGGGTTTAACTCGAGTTAAACCCTTGGAGGAGGCAC-3'

Other clones with same target sequence:

(none)