Construct: shRNA TRCN0000359951

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001080426.1-3693s21c1
DNA Barcode:
GCGGACCAACTGATACCATTT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
DUSP27 (92235)
Gene Description:
dual specificity phosphatase 27, atypical
Transcript:
RefSeq NM_001080426.1 (NON-CURRENT)
Match location:
Position 3693 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000359951)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCGGACCAACTGATACCATTT
Hairpin Sequence:
5'-CCGG-GCGGACCAACTGATACCATTT-CTCGAG-AAATGGTATCAGTTGGTCCGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCGGACCAACTGATACCATTTCTCGAGAAATGGTATCAGTTGGTCCGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCGGACCAACTGATACCATTTCTCGAGAAATGGTATCAGTTGGTCCGC-3'

Other clones with same target sequence:

(none)