Construct: shRNA TRCN0000359992

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_025179.3-953s21c1
DNA Barcode:
GGCCATCAACCGGGTCTATAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
PLXNA2 (5362)
Gene Description:
plexin A2
Transcript:
RefSeq NM_025179.3 (NON-CURRENT)
Match location:
Position 953 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000359992)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GGCCATCAACCGGGTCTATAA
Hairpin Sequence:
5'-CCGG-GGCCATCAACCGGGTCTATAA-CTCGAG-TTATAGACCCGGTTGATGGCC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGGCCATCAACCGGGTCTATAACTCGAGTTATAGACCCGGTTGATGGCCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGGCCATCAACCGGGTCTATAACTCGAGTTATAGACCCGGTTGATGGCC-3'

Other clones with same target sequence:

(none)