Construct: shRNA TRCN0000360087

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_013263.2-1436s21c1
DNA Barcode:
TATGTCATGGCAGATAGTTTA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
BRD7 (29117)
Gene Description:
bromodomain containing 7
Transcript:
RefSeq NM_013263.2 (NON-CURRENT)
Match location:
Position 1436 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000360087)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TATGTCATGGCAGATAGTTTA
Hairpin Sequence:
5'-CCGG-TATGTCATGGCAGATAGTTTA-CTCGAG-TAAACTATCTGCCATGACATA-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTATGTCATGGCAGATAGTTTACTCGAGTAAACTATCTGCCATGACATATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATATGTCATGGCAGATAGTTTACTCGAGTAAACTATCTGCCATGACATA-3'

Other clones with same target sequence:

(none)