Construct: shRNA TRCN0000360108

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_020920.2-2742s21c1
DNA Barcode:
TAGACATCCTAGAGGATTATT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
CHD8 (57680)
Gene Description:
chromodomain helicase DNA binding protein 8
Transcript:
RefSeq NM_020920.2 (NON-CURRENT)
Match location:
Position 2742 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000360108)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TAGACATCCTAGAGGATTATT
Hairpin Sequence:
5'-CCGG-TAGACATCCTAGAGGATTATT-CTCGAG-AATAATCCTCTAGGATGTCTA-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTAGACATCCTAGAGGATTATTCTCGAGAATAATCCTCTAGGATGTCTATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATAGACATCCTAGAGGATTATTCTCGAGAATAATCCTCTAGGATGTCTA-3'

Other clones with same target sequence:

(none)