Construct: shRNA TRCN0000360131

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_017881.2-576s21c1
DNA Barcode:
ATATGGAATAGAAGCTATTTC

Original Target:

Taxon:
Homo sapiens (human)
Gene:
NMRK1 (54981)
Gene Description:
nicotinamide riboside kinase 1
Transcript:
RefSeq NM_017881.2 (NON-CURRENT)
Match location:
Position 576 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000360131)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
ATATGGAATAGAAGCTATTTC
Hairpin Sequence:
5'-CCGG-ATATGGAATAGAAGCTATTTC-CTCGAG-GAAATAGCTTCTATTCCATAT-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGATATGGAATAGAAGCTATTTCCTCGAGGAAATAGCTTCTATTCCATATTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAATATGGAATAGAAGCTATTTCCTCGAGGAAATAGCTTCTATTCCATAT-3'

Other clones with same target sequence:

(none)