Construct: shRNA TRCN0000360149

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_002351.3-861s21c1
DNA Barcode:
GCATCTAGCATGTCGTCAAAG

Original Target:

Taxon:
Homo sapiens (human)
Gene:
SH2D1A (4068)
Gene Description:
SH2 domain containing 1A
Transcript:
RefSeq NM_002351.3 (NON-CURRENT)
Match location:
Position 861 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000360149)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCATCTAGCATGTCGTCAAAG
Hairpin Sequence:
5'-CCGG-GCATCTAGCATGTCGTCAAAG-CTCGAG-CTTTGACGACATGCTAGATGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCATCTAGCATGTCGTCAAAGCTCGAGCTTTGACGACATGCTAGATGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCATCTAGCATGTCGTCAAAGCTCGAGCTTTGACGACATGCTAGATGC-3'

Other clones with same target sequence:

(none)