Construct: shRNA TRCN0000360454

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_019730.2-319s21c1
DNA Barcode:
ATGCTTCGCGAGCCCTCATAG

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Nme3 (79059)
Gene Description:
NME/NM23 nucleoside diphosphate kinase 3
Transcript:
RefSeq NM_019730.2 (CURRENT)
Match location:
Position 319 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000360454)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
ATGCTTCGCGAGCCCTCATAG
Hairpin Sequence:
5'-CCGG-ATGCTTCGCGAGCCCTCATAG-CTCGAG-CTATGAGGGCTCGCGAAGCAT-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGATGCTTCGCGAGCCCTCATAGCTCGAGCTATGAGGGCTCGCGAAGCATTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAATGCTTCGCGAGCCCTCATAGCTCGAGCTATGAGGGCTCGCGAAGCAT-3'

Other clones with same target sequence:

(none)