Construct: shRNA TRCN0000360495

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_181322.3-1677s21c1
DNA Barcode:
GCGAAAGCAGCATTCCTATAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Ctcf (13018)
Gene Description:
CCCTC-binding factor
Transcript:
RefSeq NM_181322.3 (CURRENT)
Match location:
Position 1677 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000360495)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCGAAAGCAGCATTCCTATAT
Hairpin Sequence:
5'-CCGG-GCGAAAGCAGCATTCCTATAT-CTCGAG-ATATAGGAATGCTGCTTTCGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCGAAAGCAGCATTCCTATATCTCGAGATATAGGAATGCTGCTTTCGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCGAAAGCAGCATTCCTATATCTCGAGATATAGGAATGCTGCTTTCGC-3'

Other clones with same target sequence:

(none)