Construct: shRNA TRCN0000360575

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_133919.3-853s21c1
DNA Barcode:
TGGCGAAGGCCCAAGATAAAG

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Aff1 (17355)
Gene Description:
AF4/FMR2 family, member 1
Transcript:
RefSeq NM_133919.3 (NON-CURRENT)
Match location:
Position 853 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000360575)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TGGCGAAGGCCCAAGATAAAG
Hairpin Sequence:
5'-CCGG-TGGCGAAGGCCCAAGATAAAG-CTCGAG-CTTTATCTTGGGCCTTCGCCA-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTGGCGAAGGCCCAAGATAAAGCTCGAGCTTTATCTTGGGCCTTCGCCATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATGGCGAAGGCCCAAGATAAAGCTCGAGCTTTATCTTGGGCCTTCGCCA-3'

Other clones with same target sequence:

(none)