Construct: shRNA TRCN0000360607

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_030612.3-1290s21c1
DNA Barcode:
CCAGTCTCCCAAGTATGATTC

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Nfkbiz (80859)
Gene Description:
nuclear factor of kappa light polypeptide gene enhancer in B cells inhibitor, zeta
Transcript:
RefSeq NM_030612.3 (CURRENT)
Match location:
Position 1290 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000360607)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCAGTCTCCCAAGTATGATTC
Hairpin Sequence:
5'-CCGG-CCAGTCTCCCAAGTATGATTC-CTCGAG-GAATCATACTTGGGAGACTGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCAGTCTCCCAAGTATGATTCCTCGAGGAATCATACTTGGGAGACTGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCAGTCTCCCAAGTATGATTCCTCGAGGAATCATACTTGGGAGACTGG-3'

Other clones with same target sequence:

(none)