Construct: shRNA TRCN0000360622

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_145067.3-2721s21c1
DNA Barcode:
ACCGACACGCGGTAGACATTT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Gucy2c (14917)
Gene Description:
guanylate cyclase 2c
Transcript:
RefSeq NM_145067.3 (CURRENT)
Match location:
Position 2721 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000360622)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
ACCGACACGCGGTAGACATTT
Hairpin Sequence:
5'-CCGG-ACCGACACGCGGTAGACATTT-CTCGAG-AAATGTCTACCGCGTGTCGGT-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGACCGACACGCGGTAGACATTTCTCGAGAAATGTCTACCGCGTGTCGGTTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAACCGACACGCGGTAGACATTTCTCGAGAAATGTCTACCGCGTGTCGGT-3'

Other clones with same target sequence:

(none)