Construct: shRNA TRCN0000360623

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_145067.3-388s21c1
DNA Barcode:
CTCCGACGGTCTGATTCATAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Gucy2c (14917)
Gene Description:
guanylate cyclase 2c
Transcript:
RefSeq NM_145067.3 (CURRENT)
Match location:
Position 388 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000360623)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CTCCGACGGTCTGATTCATAA
Hairpin Sequence:
5'-CCGG-CTCCGACGGTCTGATTCATAA-CTCGAG-TTATGAATCAGACCGTCGGAG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCTCCGACGGTCTGATTCATAACTCGAGTTATGAATCAGACCGTCGGAGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACTCCGACGGTCTGATTCATAACTCGAGTTATGAATCAGACCGTCGGAG-3'

Other clones with same target sequence:

(none)