Construct: shRNA TRCN0000360701

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_011160.2-2431s21c1
DNA Barcode:
CCTTGCTTTGCTCTGATTATA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Prkg1 (19091)
Gene Description:
protein kinase, cGMP-dependent, type I
Transcript:
RefSeq NM_011160.2 (NON-CURRENT)
Match location:
Position 2431 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000360701)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCTTGCTTTGCTCTGATTATA
Hairpin Sequence:
5'-CCGG-CCTTGCTTTGCTCTGATTATA-CTCGAG-TATAATCAGAGCAAAGCAAGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCTTGCTTTGCTCTGATTATACTCGAGTATAATCAGAGCAAAGCAAGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCTTGCTTTGCTCTGATTATACTCGAGTATAATCAGAGCAAAGCAAGG-3'

Other clones with same target sequence:

(none)