Construct: shRNA TRCN0000360714

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001085410.1-536s21c1
DNA Barcode:
ACTACTTGAGCGACATCATAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Nadk2 (68646)
Gene Description:
NAD kinase 2, mitochondrial
Transcript:
RefSeq NM_001085410.1 (NON-CURRENT)
Match location:
Position 536 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000360714)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:


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Sequence Information

Target Sequence:
ACTACTTGAGCGACATCATAT
Hairpin Sequence:
5'-CCGG-ACTACTTGAGCGACATCATAT-CTCGAG-ATATGATGTCGCTCAAGTAGT-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGACTACTTGAGCGACATCATATCTCGAGATATGATGTCGCTCAAGTAGTTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAACTACTTGAGCGACATCATATCTCGAGATATGATGTCGCTCAAGTAGT-3'

Other clones with same target sequence:

(none)