Construct: shRNA TRCN0000360759

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_198164.2-830s21c1
DNA Barcode:
CTGTCGTCAGGAGGATATAAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Cdk19 (78334)
Gene Description:
cyclin-dependent kinase 19
Transcript:
RefSeq NM_198164.2 (NON-CURRENT)
Match location:
Position 830 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000360759)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CTGTCGTCAGGAGGATATAAA
Hairpin Sequence:
5'-CCGG-CTGTCGTCAGGAGGATATAAA-CTCGAG-TTTATATCCTCCTGACGACAG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCTGTCGTCAGGAGGATATAAACTCGAGTTTATATCCTCCTGACGACAGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACTGTCGTCAGGAGGATATAAACTCGAGTTTATATCCTCCTGACGACAG-3'

Other clones with same target sequence:

(none)