Construct: shRNA TRCN0000360767

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001033209.2-2329s21c1
DNA Barcode:
TTCATCTTCCCTCCCTATATG

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Xylb (102448)
Gene Description:
xylulokinase homolog (H. influenzae)
Transcript:
RefSeq NM_001033209.2 (NON-CURRENT)
Match location:
Position 2329 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000360767)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TTCATCTTCCCTCCCTATATG
Hairpin Sequence:
5'-CCGG-TTCATCTTCCCTCCCTATATG-CTCGAG-CATATAGGGAGGGAAGATGAA-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTTCATCTTCCCTCCCTATATGCTCGAGCATATAGGGAGGGAAGATGAATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATTCATCTTCCCTCCCTATATGCTCGAGCATATAGGGAGGGAAGATGAA-3'

Other clones with same target sequence:

(none)