Construct: shRNA TRCN0000360784

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001145854.1-1502s21c1
DNA Barcode:
ACGCAGGGAGCTGCTCTTTAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Pak6 (214230)
Gene Description:
p21 protein (Cdc42/Rac)-activated kinase 6
Transcript:
RefSeq NM_001145854.1 (CURRENT)
Match location:
Position 1502 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000360784)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
ACGCAGGGAGCTGCTCTTTAA
Hairpin Sequence:
5'-CCGG-ACGCAGGGAGCTGCTCTTTAA-CTCGAG-TTAAAGAGCAGCTCCCTGCGT-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGACGCAGGGAGCTGCTCTTTAACTCGAGTTAAAGAGCAGCTCCCTGCGTTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAACGCAGGGAGCTGCTCTTTAACTCGAGTTAAAGAGCAGCTCCCTGCGT-3'

Other clones with same target sequence:

(none)