Construct: shRNA TRCN0000360802

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001033285.1-1111s21c1
DNA Barcode:
AGCCCGACAGATACGTCAAAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Cdc42bpa (226751)
Gene Description:
CDC42 binding protein kinase alpha
Transcript:
RefSeq NM_001033285.1 (CURRENT)
Match location:
Position 1111 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000360802)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
AGCCCGACAGATACGTCAAAT
Hairpin Sequence:
5'-CCGG-AGCCCGACAGATACGTCAAAT-CTCGAG-ATTTGACGTATCTGTCGGGCT-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGAGCCCGACAGATACGTCAAATCTCGAGATTTGACGTATCTGTCGGGCTTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAAGCCCGACAGATACGTCAAATCTCGAGATTTGACGTATCTGTCGGGCT-3'

Other clones with same target sequence:

(none)