Construct: shRNA TRCN0000360808

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_027184.1-777s21c1
DNA Barcode:
GATGCGATTGCCGCCAGTATT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Ipmk (69718)
Gene Description:
inositol polyphosphate multikinase
Transcript:
RefSeq NM_027184.1 (NON-CURRENT)
Match location:
Position 777 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000360808)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GATGCGATTGCCGCCAGTATT
Hairpin Sequence:
5'-CCGG-GATGCGATTGCCGCCAGTATT-CTCGAG-AATACTGGCGGCAATCGCATC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGATGCGATTGCCGCCAGTATTCTCGAGAATACTGGCGGCAATCGCATCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGATGCGATTGCCGCCAGTATTCTCGAGAATACTGGCGGCAATCGCATC-3'

Other clones with same target sequence:

(none)